Regional Centre, Hyderabad was established in 1989 and is located on the premises of CCMB, Hyderabad. This centre has been regularly supplying ready-to-use Sodium pertechnetate extracted from Molybdenum-99 (99Mo) using the Automated Solvent Extraction (AUTOSOLEX) Generator, indigenously developed by BRIT, Vashi and VECC, Kolkata.
Regulatory permission for procurement of 99Mo/99mTc and 68Ge/68Ga generators was recently received from AERB and this will help cater to the increasing demand by Nuclear Medicine centres located at Hyderabad for SPECT and PET diagnostic imaging using ready-to-use 99mTc- and 68Ga-radiopharmaceuticals.
This centre serves as a retail outlet for sale of TCK cold kits for the preparation of 99mTc- radiopharmaceutical formulations and will soon introduce supply of deuterated solvents in this mode.
Synthesis and supply of Phosphorous-32 (32P) labelled nucleotides to leading research institutes, universities and industrial research centres has been a notable service offered by this centre since the last several years. These nucleotides find application in the area of in vitro kinase assays, generation of labelled DNA/RNA probes for hybridization, DNA fingerprinting, sequencing etc. and offer reliability and excellence in performance on par with the highest international standards. The labelled nucleotides are available in two different formulations (under LCP and PLC series) and shipped in dry ice and at ambient temperature, respectively. The centre produces and supplies a variety of Molecular Biology reagents and kits viz. Taq DNA polymerase, PCR master mix, nucleic acid isolation and DNA labelling kits for application in the areas of healthcare, agriculture, Life Sciences and Biotechnology research.
Real-time PCR applications for molecular diagnostics has been an additional major research area and has contributed significantly towards improvement in products and technology development.
This Centre is involved in Production of :
Code Product TCM 6 Ready-to-use [99mTc]NaTcO4 (Sodium pertechnetate) in physiological solution
Code Product LCP 101/PLC 101 [ɣ 32P] ATP - Adenosine 5’ [ɣ 32P] triphosphate LCP 102/PLC 102 [α 32P] dCTP-Deoxycytidine 5'[α 32P] triphosphate LCP 103/PLC 103 [α 32P] dATP-Deoxyadenosine 5'[α 32P] triphosphate LCP 104/PLC 104 [α 32P] dGTP-Deoxyguanosine 5'[α 32P] triphosphate LCP 106/PLC 106 [α 32P] ATP - Adenosine 5'[α 32P] triphosphate LCP 107/PLC 107 [α 32P] GTP - Guanosine 5'[α 32P] triphosphate LCP 108/PLC 108 [α 32P] UTP - Uridine 5'[α 32P] triphosphate LCP 109/PLC 109 [α 32P] CTP - Cytidine 5'[α 32P] triphosphate LCP 1010/PLC 1010 [α 32P] PCP - Cytidine 3', 5', [α 32P] biphosphate LCP 1011/PLC 1011 [α32P] GTP - Guanosine 5'[α 32P] triphosphate LCP 32 [32P] - Orthophosphoric Acid
LCP formulation is shipped in dry ice and PLC formulation in ambient temperature according to the dispatch schedule.
- MOLECULAR BIOLOGY REAGENTS
Code Product LCE 101 Taq DNA Polymerase (100 to 1000 Units) LCE 102 Taq DNA Polymerase (1001 to 4000 Units) LCE 103 Taq DNA Polymerase (4001 to 50000 Units) LCE 104 Taq DNA Polymerase (50001- 1 Lac Units) LCE 105 Taq DNA Polymerase (above 1 Lac units) LCK 1601 dNTP set for PCR (1 X 25 µl of dATP, dCTP, dGTP, dTTP-100mM each) LCK 1602 dNTP set for PCR (3 X 25 µl of dATP, dCTP, dGTP, dTTP-100mM each) LCK 1603 dNTP set for PCR (5 X 25 µl of dATP, dCTP, dGTP, dTTP-100mM each) LCK 1604 dNTP set for PCR (10X25 µl of dATP, dCTP, dGTP, dTTP-100mM each) PMX 01 PCR Master Mix (100 Reactions) PMX 02 PCR Master Mix (250 Reactions) PMX 05 PCR Master Mix (500 Reactions) PMX 10 PCR Master Mix (1000 Reactions) PMX 1000 >PCR Master Mix (Bulk requirement; please enquire)
- MOLECULAR BIOLOGY KITS
Code Product LCK-1 Nick Translation Kit (20 reactions) LCK-2 Random Primer Labeling Kit (30 reactions) LCK-3 5’ End Labeling kit (10 reactions) LCK 20/21 Genomic DNA Isolation kit (50/100 purifications) LCK 22/23 DNA Isolation kit (Plasmid) (50/100 purifications) LCK 24/25 DNA Gel Purification kit (50/100 purifications) LCK 26/27 PCR Product Purification kit (50/100 purifications)